Analysis of Field Potential Parameters in iCell Cardiomyocytes Using the CFPS-32™ System
A total of 14,000–50,000 cardiomyocytes per well were cultured on CFPS-ITO-16W2E cell chip up to DIV28, and field potentials were measured using the CFPS-32TM system. (A) The key field potential parameters of cardiomyocytes were analyzed using the CFPS-32 software. (B) Cardiomyocyte beat period stabilized between 1-2 seconds starting from DIV6. (C) A consistent BPM around 30-60 was observed across various densities. (D) While the spike amplitude generally showed high variability, it tended to increase with higher cell densities, and after DIV8, it remained above 0.3 mV. (E) FPDcf stabilized around DIV10 and tended to increase with longer incubation periods. All parameters were the most stable between DIV10-12. The red dotted lines indicate the acceptable range for each parameter.
Cellames Cell Analysis Team